#Biomarker Study
Biomarker finding by the analysis of blood, urine, or other samples, by genomics, transcriptomics, proteomics, metabolomics, lipidomics, glycomics, and secretomics
patterns can identify tens, hundreds and even thousands of genes and compounds that correlate with disease state.
Genome Landscapes (instead of single gene testing) allow multiple genes to be analyzed simultaneously from the same tissue sample
Protein analysis
Activity-based protein profiling (analyzing lysates)
Labeled lysate with the FP-ABP, added a biotin tag by click chemistry
Purified enzymes through streptavidin affinity chromatography
Washed and performed on-resin trypsin digest
Analyzed by high-resolution LC-MS
Genotyping using commercial Line Probe Assay (Innogenetics) , confirmed by sequencing of Core PCR products followed by phylogenetic analysis
Biomarker finding by the analysis of blood, urine, or other samples, by genomics, transcriptomics, proteomics, metabolomics, lipidomics, glycomics, and secretomics
patterns can identify tens, hundreds and even thousands of genes and compounds that correlate with disease state.
Non-invasive testing is preferred now-a-days
Blood based genomic/ molecular diagnostic test for patients in an age of personalized medicine (e.g. small blood sample from the patient; high-throughput processing in a centralized laboratory; genome, exome, and transcriptome; protein expression information)
Tests are being developed and submitted to FDA for approval
Host factors (Genes related to cell death, cell signaling, and DNA damage repair) and tolerance (radiation dose, drugs dose)
Multiple genes are activated in circulating blood cells in patients (CRP protein, prolactin, insulin, cortisol etc.)
Gene expression score (GES) diagnostic test can analyze gene expression for diagnosis of inflammatory disease, arterial blockage
Peaks (amplified genomic regions) and valleys (deleted genomic regions)Genome Landscapes (instead of single gene testing) allow multiple genes to be analyzed simultaneously from the same tissue sample
Protein analysis
Activity-based protein profiling (analyzing lysates)
Labeled lysate with the FP-ABP, added a biotin tag by click chemistry
Purified enzymes through streptavidin affinity chromatography
Washed and performed on-resin trypsin digest
Analyzed by high-resolution LC-MS
Genotyping using commercial Line Probe Assay (Innogenetics) , confirmed by sequencing of Core PCR products followed by phylogenetic analysis
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